Maria Stuarda

Magda Vášáryová

Mary Stuart

Joan Sutherland

Mary Stuart (singing voice)

Kamila Magálová

Queen Elizabeth I

Huguette Tourangeau

Queen Elizabeth I (singing voice)

Milan Kňažko

Earl of Leicester

Luciano Pavarotti

Earl of Leicester (singing voice)

Přemysl Kočí

George Talbot

Roger Soyer

George Talbot (singing voice)

Jan Přeučil

William Cecil

James Morris

William Cecil (singing voice)

Jiřina Jirásková

Hanna Kennedy

Margreta Elkins

Hanna Kennedy (singing voice)

Luděk Munzar

Amias Paulet

Vladimír Dlouhý

Mortimer

Karel Roden

William Davison

Jiří Vala

Melville

Zdeněk Ornest

Kent (uncredited)

Kornelia Boje

Mary Stuart (voice)